design patterns - Spring Dependency injection injection level -

i new @ spring.recently doing major project on fuzzy based decision support system.it web based application.i using various spring frameworks in project spring security ,springjdbc,and dependency injection , spring mvc. my quesation level dependency injection can done..? want elaborate it... suppose have class defines request scope bean in spring configuration file .i have singleton daoimpl bean of dependency above request scope bean.finally daoimpl bean autowired singleton controller.with set app works fine.here dependency injected upto 2 level first request scope bean daoimpl beans , daoimpl bean controller. in same pattern level dependency can injected in spring web.i m talking inejcting session , scope beans ohter scope bean on spring. appricated.